| 摘要 |
PURPOSE:To carry out culture in the same apparatus consistently from a material to be cultivated to histodifferentiation, by adjusting an angle of inclination of a culture medium, the intensity of irradiation, etc., and a gas atmosphere composition by a gas atmosphere controlling means to optimum conditions depending upon each process of culture. CONSTITUTION:A culture device 13 having plural culture tubes 14 is set rotatably by a rotary drive motor 15 and can be laid at any angle of inclination from a horizontal state to a slant state on a rotary table 12 in a constant temperature tank 11 laid in a culture apparatus 10 and an optical system 16 equipped with plural lamps 17 which are fixed in each group unit to control the intensity of irradiation is set. Then a material to be cultivated and a culture solution are put in the culture tubes 14, temperature, illuminance, an angle of inclina tion of the culture medium 13 and number of revolutions are adjusted to optimum states depending upon each process of culture. An air feed source 22, an N2 gas feed source 23 and a CO2 gas fed source 24 are connected through lines L1-3, respectively, to a piping storing part 21b of a control device 21, the air feed source is connected to a joint terminal 26, the N2 gas feed source and the CO2 gas feed source are connected to solenoid valves 28 and 29 and from a common line L4 to a joint terminal 27. On the other hand, the solenoid valves 28 and 29 are connected through a common line L5 to a control part 21a and feed of gases are controlled through a gas sensor in the constant temperature bath tank 11 to adjust a gas atmosphere composition to optimum condition.
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