| 摘要 |
PURPOSE:To quantify hypoxanthine and inosine continuously, rapidly and simply, by using not only an immobilized enzyme membrane excellent in reaction selectivity and a material exclusion property but also a hydrogen peroxide electrode method. CONSTITUTION:Xanthine oxidase is allowed to act on hypoxanthine to convert the same to uric acid using an enzyme electrode for detecting uric acid constituted of an immobilized uricase membrane 20 and a hydrogen peroxide electrode 21 while nucleoside phospholylase and xanthine oxidase are allowed to act on inosine to convert the same to uric acid and the concns. of uric acid produced corresponding to the concn. of hypoxanthine and that of inosine are respectively detected by the enzyme electrode 10, and hypoxanthine and inosine are quantified from the output current obtained. By this method, without requiring complicated operation such as deproteinizing treatment, separation or fractionation, inosine and hypoxanthine can be measured accurately, simply, rapidly and inexpensively. |
