发明名称 |
Process for the preparation of L-phosphinothricine by transamination. |
摘要 |
Prodn. of L-tert.-leucine (Ia) or L-phosphinothricin (Ib) is effected by tranamination of 3,3-dimethyl-2-oxobutyric acid (IIa) or (3-carboxy-3-oxopropyl)methylphosphinic acid (IIb), or their salts, with an amino acid (III) as amino gp. binder. Pref. the reaction is effected in the presence of a transaminase enzyme at pH 7-8.5 using a (III):(II) ratio of 1-5:1, esp. 1-2:1. The enzyme is used in pure form, in the form of a microorganism, or in the form of a cell extract or protein isolate derived from a microorganism. The microorganism is a Paracoccus, Alcaligenes, Pseudomonas, Serratia, Agrobacterium, Streptomyces or Enterbacterium strain, or a genetically manipulated microorganism, esp. E.coli ATCC 11303 transformed with a plasmid contg. a tyrB or 1lvE gene. |
申请公布号 |
EP0248357(A2) |
申请公布日期 |
1987.12.09 |
申请号 |
EP19870107793 |
申请日期 |
1987.05.29 |
申请人 |
HOECHST AKTIENGESELLSCHAFT |
发明人 |
THEN, JOHANN, DR.;BARTSCH, KLAUS, DR.;DEGER, HANS-MATTHIAS, DR.;GRABLEY, SUSANNE, DR.;MARQUARDT, RUDIGER, DR. |
分类号 |
C12N15/09;C12N1/20;C12N1/21;C12N9/10;C12N15/00;C12N15/54;C12P13/04;C12P13/06;C12R1/01;C12R1/05;C12R1/185;C12R1/19;C12R1/38;C12R1/425;C12R1/465 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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