METHOD FOR PRODUCING MONOCLONAL ANTIBODIES TO AN IMMUNOGENIC ANTIBODY-ANTIGEN CONJUNCTION

摘要

A method for the preparation of a monoclonal antibody capable of recognizing the juncture of an antigenic determinant and a corresponding antibody binding site when complexed to each other. Antigen may be subjected to limited proteolytic digest to obtain an immunochemically active fragment. Similarly, a monoclonal antibody directed against the antigenic determinant of interest is enzymatically cleaved to obtain the Fab or F(ab')2 fragment. The two fragments are combined so as to form a complex. This immunogenic complex is subsequently injected into recipient animal and the antibodies produced recognize specifically the juncture of the immunogenic complex.