摘要 |
PURPOSE:To efficiently obtain the titled compound, by reacting an N-protected amino acid with an amino acid in an aqueous layer in a two-phase system reaction medium of an organic solvent containing a quaternary ammonium salt, etc., and water using a proteolytic enzyme and migrating the resultant product to an organic solvent phase. CONSTITUTION:An N-protected amino acid, e.g. N-acetyl-L-aspartic acid, etc., or a derivative thereof is reacted with an amino acid, e.g., L-phenylalanine, etc., or a derivative thereof in an aqueous layer in a two-phase system reaction medium of a water-immiscible organic solvent containing a dissolved quaternary ammonium salt, e.g. trioctylmethylammonium chloride, etc., or quaternary phosphonium salt and water using a proteolytic enzyme to form an N-protected peptide containing one or more carboxyl groups. The resultant N-protected peptide is then converted into a lipophilic quaternary ammonium salt or phosphonium salt and migrated to the organic solvent phase to afford the aimed compound.
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