| 摘要 |
PURPOSE:To enable efficient production of HBsAg containing Pre S region and expected to have high immunogenicity inhuman body, by using a miniaturized improved yeast promoter. CONSTITUTION:A GAP-DH protein is cut with a restriction enzyme XmnI at a part near -164 bp of initiation codon and further cut at upper stream -25 bP or thereabout with HindIII and the product is subjected to depletion treatment to obtain a miniaturized promoter having a DNA sequence of formula. The promoter is used as a yeast glyceroaldehyde-3-phosphate dehydrogenase (GAP-DH) promoter. The objective Pre S-HBsAg can be produced by using the above promoter, a PBR322 derivative as a basic vector and a yeast Saccharomyces cerevisiae as a host. The DNA recombination operation can be simplified by the use of the miniaturized promoter, the hybridization of promoter is facilitated and the production of a promoter in improved productivity can be expected. |
