Immunological method involving formation of ¬anti-receptor:anti-ligand antibody complex

摘要

In a immunological method antibodies are produced which are capable of reacting with a specific part of a contact surface of either of two macromolecules interacting to form a complex, the method comprising a) separately producing polyclonal antisera containing antibodies against the respective macromolecules, b) contacting the immunoglobulin fractions of said antisera with each other, such that complementary antibodies may react to form antibody complexes, c) separating the pairs of complexed antibodies obtained from antibodies which have not reacted with each other, and d) dissociating said antibody complexes and separately recovering at least one antibody component of said antibody complexes. The antibodies obtained may be used for identifying and recovering antigens or fragments thereof from a mixture or as substitute antigens.