| 摘要 |
<p>A method of detecting the presence of a cell, virus or circulating body component or an antibody thereto in a sample comprises contacting the sample with a regent bound to a solid support, usually a polymer. The reagent may for instance be a carbohydrate, a protein or an antibody. Having been contacted with the sample, the reagent bound to the solid support is contacted with the same or another reagent coupled to a macromolecular wtaer-insoluble carrier, for instance polymeric microbeads, to which is further multivalently bound a substance which is capable of initiating a reaction having a detectable reaction product. Alternatively, the reagent is coupled to the substance multivalently bound to the carrier. The substance may be an enzyme, antibody or blood clotting factor. Then, the reagent bound to the solid support is reacted with a compound which is a precursor of a detectable reaction product or capable of initiating a reaction cascade the end product of which is detectable. The compound may be an enzyme substrate, an antigen or a blood clotting factor. If any sample component has become bound to the reagent, a detectable reaction product is formed.</p> |
