MONOCLONAL ANTIBODY AGAINST OUTER MEMBRANE F MONOMER ORIGINATED FROM E.COLI AND HYBRIDOMA PRODUCING SAME

摘要

PURPOSE:To produce a monoclonal antibody capable of specifically recognizing a specific peptide, by forming a hybridoma producing a monoclonal antibody reactive with an outer membrane protein F monomer (OmpF) originated from E.coli. CONSTITUTION:A spleen cell of a mouse immunized with OmpF is fused with a mouse myeloma cell and cultured in a selective medium containing biosynthesis-inhibiting agent to obtain a fused cell. The antibody activity in the supernatant culture liquid is screened by an immunological method to form a cell strain producing an antibody having specific reactivity to OmpF and the strain is cloned to obtain a hybridoma. The hybridoma is inoculated on a plate for tissue culture and cultured in a serum-free medium or a serum- containing medium for animal cell culture to obtain a monoclonal antibody 7B12/4E3, etc., reactive with the outer membrane protein monomer originated from E.coli.