| 摘要 |
A device for use in the detection, in a liquid sample, of a predetermined analyte which is reactive under predetermined enzyme catalysed conditions so as to produce at least one of a proton and hydrogen peroxide. The device comprises an electrically conducting polymer element (2) having spaced apart connections (9) to an electrical circuit means (12) for directly or indirectly detecting changes in electrical resistance in the element (2). The polymer (2) contains conjugatable monomer units and is permeable to protons and where said analyte under said enzyme catalyzed conditions produces hydrogen peroxide, to hydrogen peroxide. The polymer (2) further has an electrical conductivity which is variable according to the amount of protons or hydrogen peroxide in contact therewith, and an enzyme support element (4) containing at least one enzyme and any cofactor(s) required in the predetermined enzyme-catalysed conditions, in an analyte permeable substantially non-conducting medium and also has a first face (5) in contact with the polymer element (2) and a second face (6) disposable in contact with the liquid sample. The present invention also provides a method of detecting a predetermined analyte in a liquid sample comprising the steps of contacting the liquid sample with the second face (6) of the enzyme support element (4) of the device and measuring the change in resistance between the connection means (9). |
