VACCINE AGAINST VIRAL HEPATITIS B, METHOD AND TRANSFORMED EUCARYOTIC CELLS FOR THE PREPARATION OF THIS VACCINE

摘要

1. A process for producing a culture of competent eucaryotic cells capable of expressing a viral DNA and selected from those which are responsible for a hepatitis, wherein the competence of the eucaryotic cells which are capable of being used can be appreciated by the capacity which can be imparted thereto, after transformation thereof with a vector containing a tandem sequence comprising two DNA HBV, to secrete an immunogenic protein capable of inducing in vivo the production of active antibodies against the entire virus of viral hepatitis B, characterised by the operation which consists of transforming said culture of eucaryotic cells with a vector, more particularly a plasmid which itself contains an insertion sequence which itself comprises at least the part of the viral DNA which codes for an immunogenic protein capable of inducing in vivo the production of active antibodies with regard to the entire virus, and the promoter of said virus, under the control of which are effected transcription and translation in the normal host infected by said virus of said part of viral DNA.