Process for identifying a colony of Rhizobium japonicum.

摘要

A process for identifying a colony of Rhizobium japonicum having hydrogen-uptake activity from a group of colonies, some of which do not have hydrogen-uptake activity, comprising: replicating colonies of the group onto a surface of a porous, sterile support material; incubating cells on the surface under conditions that derepress their hydrogenase; treating the support material with a methylene blue indicator solution containing inhibitors of endogenous respiration; maintaining the support material with attached colonies in an H2 atmosphere whereupon colonies with hydrogen uptake activity reduce the methylene blue to its leuco form.