Process and reagent for the determination of alpha -amylase

摘要

A process and reagent are described for the determination of alpha -amylase by enzymatic splitting of an alpha -amylase substrate in the presence of alpha -glucosidase and beta -glucosidase and measurement of a fission product. The substrate utilized is a 4-nitrophenyl- beta ,D-maltoheptaoside substituted in the phenyl ring by an electron withdrawing group, preferably a 2-chloro group.