摘要 |
A method of producing and packaging a two-part or multiple-part polyacrylamide gel with a common buffer for electrophoresis, comprising the steps of casting within an upright narrow container, a stacking gel part followed, after polymerisation, with a resolving gel part. When the gel system is complete a part of the container is removed leaving the assembly on a backing sheet. The exposed upper surface of the gel is then covered by a protective film, and a plurality of gels fabricated in this manner may be stacked in superimposed relationship and packaged for storage. The gel system is fabricated using a single buffer for the stacking and resolving gel parts which may thus be contiguous when packaged. The totally pre-cast gel has a long shelf life and its fabrication produces minimum distorsion. |