ANTI-IDIOTYPIC VACCINE FOR COCCIDIOSIS

摘要

Mouse hybridomas are developed which secrete monoclonal antibody specifically reactive with surface immunogens of Eimeria sporozoites. The monoclonal antibodies are used as immunogens in both rabbits and chickens to produce anti-idiotypic antibodies. The anti-idiotypic antibody is specific for the antigen-binding-site-associated idiotype of the monoclonal antibody, has the structural mirror image of the monoclonal antibody binding site, and is able to function as a surrogate immunogen for Eimeria. Immunization of young chickens with the anti-idiotypic antibody induces a protective immune response against a subsequent Eimeria challenge infection. The anti-idiotypic vaccine produces anti-Eimeria sporozoite antibodies, along with a reduction in cecal lesions and oocyst production following challenge with viable Eimeria sporulated oocysts.