发明名称 PLASMIDS, METHODS FOR CONSTRUCTION OF THE SAME, MICROORGANISMS CARRYING THE PLASMIDS AND METHODS FOR CULTIVATION OF THE MICROORGANISM
摘要 <p>Specification Title of the Invention Plasmids, Methods for Construction of the same, Microorganisms Carrying the Plasmids and Methods for Cultivation of the Microorganism Novel plasmids pEAP2 and pCX311, which were constructed from Bacillus sp. chromosomal DNA carrying the gene for extracellular production of such high-molecular substances as penicillinase and xylanase, and a vector plasmid pMB9 or pBR322. A novel microorganism, Escherichia coli HB101 carrying the plasmid pEAP2 ¢Escherichia coli HB101 (pEAP2)! and being capable of extracellular production of penicillinase. A novel microorganism, Escherichia coli HB101 carrying the plasmid pCX311 ¢Excherichia coli HB101 (pCX311)! and being capable of extracellular production of xylanase. Methods for cultivation of the microorganisms characterized by culturing them in the medium containing NaCl (or KCl), or NaCl (or KCl) and bran, or NaCl (or KCl) and xylan for 12-48 hours.</p>
申请公布号 CA1226833(A) 申请公布日期 1987.09.15
申请号 CA19840449095 申请日期 1984.03.08
申请人 RIKAGAKU KENKYUSHO 发明人 HORIKOSHI, KOKI;KUDO, TOSHIAKI;KATO, CHIAKI;HONDA, HIROSHI
分类号 C12N1/38;C12N9/24;C12N9/86;C12N15/70;(IPC1-7):C12N15/00;C12N1/20 主分类号 C12N1/38
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