| 摘要 |
PURPOSE:To efficiently produce creatinase without adding creatine, creatinine, etc., to a culture medium, by cultivating a strain of the genus Escherichia transformed by a gene coding creatinase. CONSTITUTION:A DNA derived from a DNA containing a gene coding creatinase, e.g. Flavobacterium U-188 (FERM-P No.2922) strain, is inserted into a vector DNA, e.g. plasmid pBR322DNA to prepare a recombinant DNA, which is used to transform or transduce a strain of the genus Escherichia, e.g. Escherichia coli HB101 (ATCC33694), etc., having the ability to produce creatinase. The resultant strain is then cultivated under aerobic condition to collect the aimed creatinase from the obtained culture. |
