发明名称 |
Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps. |
摘要 |
<p>There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.</p> |
申请公布号 |
EP0236069(A2) |
申请公布日期 |
1987.09.09 |
申请号 |
EP19870301669 |
申请日期 |
1987.02.25 |
申请人 |
CETUS CORPORATION |
发明人 |
JOHNSON, LARRY JAMES;LEATH, RICHARD ALAN;MEZEI, LOUIS MICHAEL;WIDUNAS, JOSEPH THOMAS;WENNBERG, TIMOTHY J. |
分类号 |
C12N15/09;B01J19/00;B01L7/00;C07H21/00;C07H21/02;C07H21/04;C12M1/00;C12N15/00;C12Q1/68;C40B40/06;C40B60/14;G01N33/50 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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