| 摘要 |
PURPOSE:To obtain a component effective in improving psychopathy and useful as a psychotropic agent, in high efficiency, by culturing and proliferating a vegetable of Hydrocotyle genus or Centella genus, its tissue or its cell by plant tissue culture technique and separating the active component from the cultured cell or tissue. CONSTITUTION:For example, a leafstalk of a plant of Hydrocotyle genus or Centella genus is thoroughly washed and sterilized successively with 70% ethanol and 10% bleaching powder solution. The sterilized leafstalk is cut to small pieces and cultured in an inorganic synthetic agar medium preferably containing a substance having auxin action. A standing culture or liquid culture is adopted for the culture on an industrial scale. The culture product is extracted with acetone by Soxlet extraction, hot immersion or cold immersion and the solvent is distilled off to obtain a concentrated extract of a component effective in improving psychopathy. The extract may be pulverized. It has excellent effect to improve various psychopathies such as neurosis, depression, nicotine poisoning, antihypnotic poisoning, etc.
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