ENHED TIL UDFOERELSE AF ENZYMBINDINGSASSAY

摘要

A unit for performing a binding assay with an enzyme- labelled reagent comprises an absorbent material and a developing solution, the absorbent material being provided with a plurality of reagent zones including an indicator reagent zone, and being capable of transporting the developing solution sequentially through each reagent zone by capillary action, and the indicator reagent zone including a reagent which can, directly or indirectly, immobilize an enzyme-labelled reagent in an amount dependent upon the assay result, and is characterized in that the developing solution includes a signal-producing substrate for the enzyme. The substrate moves slower through the absorbent material than the enzyme-labelled reagent or any compound of the enzyme-labelled reagent formed in the assay. The absorbent material is suitably in the form of an elongate strip provided with transverse reagent zones. The unit can be used for performing immunoassays including immunometric assays and dual analyte assays. <IMAGE>