发明名称 |
HUMAN ANTI RHESUS D PRODUCING HETEROHYBRIDOMAS |
摘要 |
<p>The present invention provides an anti-Rhesus D (RhD) monoclonal antibody producing heterohybridoma formed by fusion of non-Ig secreting mouse myeloma cells with an anti-RhD Ig producing population of Epstein Barr virus (EBV)-transformed human B lymphocytes, which heterohybridoma when cultured at 37 DEG C in hybridoma culture medium (RPMI-l640 supplemented with l0% (v/v) fetal calf serum or a medium which sustains equal growth under the same conditions) at about l x l0<6> cells/ml produces more than 20 mu g anti-RhD monoclonal antibody/ml/24hrs. Two heterohybridomas which produce IgM anti-RhD monoclonal antibodies, designated MAD-2 and FOM-l, have been deposited at the European Collection of Animal Cell Cultures, UK under accession nos. 86041803 and 87021301.</p> |
申请公布号 |
GR870644(B) |
申请公布日期 |
1987.08.28 |
申请号 |
GR19870100644 |
申请日期 |
1987.04.24 |
申请人 |
CENTRAL BLOOD LABORATORIES AUTHORITY |
发明人 |
HUGHES -JONES NEVIN CAMPBELL;THOMPSON KEITH MICHAEL;MELAMED MARK DAVID |
分类号 |
G01N33/53;A61K38/00;A61K39/395;C07K14/005;C07K14/195;C07K16/00;C07K16/34;C12N5/00;C12N5/10;C12N15/00;C12N15/02;C12P21/00;C12P21/08;C12R1/91;G01N33/577 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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