HIGH SENSITIVITY QUANTITATIVE DETERMINATION OF CRP

摘要

PURPOSE:To improve specificness and measurement sensitivity and to make quantitative determination of a low concn. of C reactive protein (CRP) by using an anti-CRP mouse monoclonal antibody as a solid phase forming antibody and enzyme labeling antibody. CONSTITUTION:The anti-CRP mouse monoclonal antibody is used both as the solid phase forming antibody and enzyme labeling antibody at the time of making quantitative determination of the CRP in a sample contg. a slight amt. of the CPR by a sandwich method. The monoclonal antibody is brought into reaction with the sample, then the activity of the labeling enzyme contributing to the antigen-antibody reaction by which the CRP is quantitatively determined. The solid phase forming antibody and enzyme labeling antibody and the CRP which is the measuring antigen can be simultaneously brought into reaction in the actual execution stage so that just one time of cleaning operation is required. The anti-CRP mouse monoclonal antibody to be used for the solid phase forming antibody and enzyme labeling antibody is preferably >=2 kinds of the monoclonal antibodies which are different from each other and conjugate each other by recognizing the respectively different antigen determination parts of the CRP which is the measuring antigen and further the antibodies which exhibit a settling reaction are more preferable.