| 摘要 |
A process for expressing proteins in Gram(-) bacteria and providing for extracellular secretion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired protein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extracellularly secreted protein; a recombinant DNA construction comprising: a promoter, a signal sequence and a structural gene including a cleavage region, wherein the structural gene is of the formula: (E)n(B)m-Y, where n is an integer >/=1, m is an integer, Y is a DNA sequence encoding a desired protein and E and B are the genes corresponding to the protein A regions E and B, with the proviso that the structural gene is different from that encoding natural protein A; a plasmid vector comprising such recombinant DNA construction; a Gram(-) bacterium harbouring said recombinant DNA construction; and proteins obtained by such process. |
