Inducible heat shock and amplification system.

摘要

The invention is directed to a method for high level polypeptide or protein production in transformed host cells. The method comprises transforming a host cell with a structural gene, encoding for the desired polypeptide or protein, operably linked to an inducible heat shock promoter. After the copy number of the structural gene is increased substantially through gene amplification, the inducible heat shock promoter is then induced to produce the polypeptide or protein.