| 摘要 |
PURPOSE:To make detection and quantitative determination of a ganglioside with an extremely slight amt. by an enzyme antigen dyeing method by separating the ganglioside in a vital sample with a thin layer chromatography then subjecting the same to a direct sialidase treatment. CONSTITUTION:After the ganglioside in the vital sample is separated with the thin layer chromatography, the sample is subjected to the direct sialidase treatment on the thin layer to conduct the ganglioside to asialoganglioside. The asialoganglioside is specifically brought into immune reaction by using the anti-asialo GM1 (GA1) or anti-asialo GM2 (GA2) antibody corresponding thereto. An enzyme labeled anti-IgG antibody is brought into reaction with the reaction complex formed by such specific immune reaction and a coloration reaction is caused with a substrate soln. after the reaction. The detection and quantitative determination of the ganglioside such as GM1 in the sample are thereby executed with the extremely slight amt.
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