| 摘要 |
<p>A new membrane lytic immunoassay. In one embodiment of this assay, namely a solution phase system, an antibody is first covalently coupled to a lipid which is to serve as an anchor for insertion into the liposome membrane. This antibody-anchor complex is used in conjunction with an otherwise non-bilayer forming lipid or mixture of lipids to form stable bilayer liposome vesicles which additionally contain a self-quenching fluorescent dye. When this antibody and dye containing liposome is brought into contact with a solution containing antigen molecules, rapid binding occurs between the antibody-anchor complex and the antigen, disrupting the liposome and releasing the dye. Release of the dye can be quantified by spectrophotometric or spectrofluorometric methods using standardized solutions.</p> |
