Method of immunochemical assay.

摘要

The present invention provides an extremely simple and effective method of a sandwich EIA (enzyme immunoassay) system, or more precisely, a method for the detection of an antigen or an antibody by means of a sandwich enzyme immunoassay system using a combined material of insoluble carrier/antigen/enzyme-labeled antibody or a combined material of insoluble carrier/antigen/enzyme-labeled antiimmunoglobulin-antibody which comprises an insoluble carrier having coated thereon a layer of an antibody or antigen specific to the antigen or antibody to be detected and a layer of an enzyme-labeled antibody or enzyme-labeled antoimmunoglobulin-antibody specific to the antigen or antibody to be detected. According to the method of the present invention, the immunoreaction product comprising fixed antibody/antigen to be detected/labeled antibody or fixed antigen/antibody to be detected/labeled antiimmunoglobulin-antibody can be formed on the surface of the insoluble carrier by only one incubation and only one washing operation.