Process for the isolation and purification of alpha-interferons

摘要

The invention relates to a process for the isolation and purification of alpha -interferons generated by plasmids from bacterial cultures which have been modified by genetic engineering, and of alpha -interferons from culture supernatants of induced mammalian cells, which comprises partition of appropriate crude substances containing interferon between an aqueous urea solution, which contains a surfactant, and a mixture of n-butanol and glacial acetic acid which contains water, and isolation of the purified alpha -interferons from the upper phase.