发明名称 |
UN METODO PARA AISLAR UNA SECUENCIA DE ACIDO NUCLEICO OBJETIVO SELECCIONADA |
摘要 |
<p>A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.</p> |
申请公布号 |
ES555984(D0) |
申请公布日期 |
1987.07.16 |
申请号 |
ES19840005559 |
申请日期 |
1986.06.12 |
申请人 |
AMGEN;ABBOTT LABORATORIES |
发明人 |
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分类号 |
G01N33/50;C07H21/04;C12N15/00;C12N15/09;C12Q1/68;C12Q1/70;G01N33/53;G01N33/532;G01N33/543;G01N33/566;(IPC1-7):G01N33/536 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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