| 摘要 |
A method for producing human leukocyte interferon alpha-2 resides in that there is carried out submerged cultivation of a producer strain Pseudomonas species VG-84 carrying a plasmid pVG3 with an inserted gene of human leukocyte interferon alpha-2, said strain being deposited in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1742; said strain being cultivated in a nutrient medium, containing the sources of carbon and nitrogen, mineral salts and growth stimulants, under aeration in the presence of antibiotics, i.e., streptomycin or tetracycline, or a mixture of both, followed by isolation and purification of the end product.
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| 申请人 |
VSESOJUZNY NAUCHNO-ISSLEDOVATELSKY INSTITUT GENETIKI |
发明人 |
DEBABOV, VLADIMIR G.;TSYGANKOV, JURY D.;CHISTOSERDOV, ANDREI J.;SVERDLOV, EVGENY D.;IZOTOVA, LARA S.;KOSTROV, SERGEI V.;STERKIN, VIKTOR E.;KUZNETSOV, VLADIMIR P.;BELYAEV, SERGEI V.;MONASTYRSKAYA, GALINA S.;SALOMATINA, IRINA S.;DOLGANOV, GRIGORY M.;ARSENIAN, SERGEI G.;TSAREV, SERGEI A.;KOZLOV, JURY I.;STRONGIN, ALEXANDR Y.;OGARKOV, VSEVOLOD I.;OVCHINNIKOV, JURY A. |
