| 摘要 |
The precision in reflectance or absorption measurements used to determine the presence and concentration of a component in an assay liquid is unexpectedly increased to essentially eliminate the variability of different reactant carriers and variability in reflectance or absorption instruments by including, either within the reactant carrier or within the assay liquid sample, in addition to a primary color forming or color changing indicator, a secondary inert colorant compound or dye having a light absorption wavelength peak separated from a light absorption wavelength peak of the primary indicator or chromogen by at least 80 nm. To achieve the full advantage of the present invention, the colorant material or marker should be inert to the reactant system in the carrier and should be inert to the assay liquid to provide a light reflectance or light absorption response essentially distinct from the light reflectance response of the primary chromogen to compensate for measurement variability. In this manner, any inaccuracy obtained from a reflectance or absorption measurement of the secondary colorant marker, incorporated into the carrier or into the assay sample in known concentration, can be used for compensation of a reflectance or absorption measurement of the primary color formed as a result of interaction or reaction of the test sample with the reagent composition, and, therefore, the concentration measurement of a predetermined component of the assay liquid. |
