| 摘要 |
PURPOSE:In treating an aqueous solution of stevioside, etc., with a specific enzyme, to produce rebaudioside A in extremely high conversion ratio while suppressing formation of by-products, by adding an organic solvent or a specific enzyme to the aqueous solution. CONSTITUTION:In converting stevioside into rebaudioside A by treating an aqueous solution containing both a beta-1,3-glucosylsaccharide compound and stevioside with an enzyme having beta-1,3-glucosyl transition activity produced by a bacterium, the aqueous solution incorporated with an organic solvent and/or a beta-1,3-glucanase of exotic type or separately treated. One or more solvents selected from acetone, ethyl acetate, methyl ethyl ketone, hexane, dimethyl sulfoxide, dioxane, thioglycol, propylene glycol, methyl phthalate, methanol and ethanol are preferable the organic solvent. |
