| 摘要 |
PURPOSE:To enable the simple high sensitivity measurement of 17alpha-hydroxyprogesterone, by preparing antigenic protein of 17alpha-hydroxyprogesterone-11- hemixasuccinate, and immunizing an animal by using this protein as an antigen to obtain anti-serum. CONSTITUTION:By using anti-serum specifically reacted with 17alpha-hydroxyprogesterone obtained by immunizing a mammal with a steriod compound represented by formula (wherein Y is antigenic protein), 17alpha-hydroxyprogesterone in blood can be measured specifically with high sensitivity. As the antigenic protein Y to be coupled, bovine serum albumin, rabbit serum albumin or egg albumin are used. In coupling, a known acid anhydride mixture method or a carbodiimide method may be used. By using this anti-serum, only 17alpha-hydroxyprogesterone can be measured with high sensitivity. By this method, the diagnosis of congenital adrenal hyperplasia or adrenal androsterone excessive formation is certainly conducted. |
