摘要 |
PURPOSE:To prepare the aimed substance, by reacting a microorganism having the ability to produce uridine diphosphate galactose and cow's milk galactosyltransferase with a substrate containing uridine monophosphate, galactose and N-acetylglucosamine. CONSTITUTION:A microorganism, e.g. Torulopsis holmii, etc., belonging to the genus Torulopsis and having the ability to produce uridine diphosphate galactose and cow's milk galactosyltransferase are simultaneously reacted with a substrate containing 5'-uridine monophosphate, galactose and N- acetylglucosamine. Thereby reaction of 5'-uridine monophosphate with galactose into uridine diphosphate galactose and reaction forming the titled substance from the above-mentioned substance and N-acetylglucosamine with cow's mild galactosyltransferase are carried out in the same system. The reactions are carried out at 4-9pH and 5-40 deg.C temperature for 2-6 days. |