| 摘要 |
PURPOSE:To produce novel chitinase capable of efficiently producing N- acetylchitooligosaccharide of pentamer or higher oligomers from N- acetylchitooligosaccharide of trimer or higher oligomer, by cultivating a microorganism, belonging to the genus Nocardia and capable of producing chitinase. CONSTITUTION:A microorganism, belonging to the genus Nocardia and capable of producing chitinase, e.g. Nocardia orientalis, is cultivated in a liquid culture medium containing preferably added chitin at 20-35 deg.C for 2-7 days preferably while shaking to collect the aimed chitinase from the cultivation filtrate. |
