Monoclonal anti-human granulocyte colony stimulating factor antibody.

摘要

A novel monoclonal anti-human granulocyte colony stimulating factor (abbreviated as G-CSF) antibody, as well as a method of purifying human G-CSF by means of using said antibody and a method of assaying the same with said antibody. Said antibody was prepared by the following steps. A laboratory animal was immunized eith human G-CSF derived from an oral cavity cancer serving as an antigen; spleen cells were extracted from the animal and fused with myeloma cells to prepare hybridomas; the hybridomas were treated to obtain clones that were capable of producing a monoclonal anti-human G-CSF antibody in high concentrations; and the clones were cultivated to obtain a monoclonal anti-human G-CSF antibody from the supernatant of the culture.