| 摘要 |
PURPOSE:To produce a large amount of mildiomycin in high efficiency on an industrial scale, by culturing a mildiomycin-producing microbial strain belonging to Streptoverticillium genus in a medium containing N-methyl compound, etc. CONSTITUTION:A mildiomycin-producing strain is converted to be resistant to serine hydroxamate or canavanine to obtain Streptomyces liquefaciens strain having improved mildiomycin-productivity and the following properties. Morphology, forms aerial mycelium; spore, oviform or cylindrical; growth temperature, 15-38 deg.C; inert to liquefaction of gelatin; assimilates inositol and does not assimilate erythritol; etc. The strain is cultured in a medium containing a carbon source, a nitrogen source, etc., and, if necessary, >=3mM of an N-methylated compound having a molecular weight of 50-1,000 or 0.1-1.0wt% 5- hydroxymethylcytosine at 15-40 deg.C for 4-14 days under slightly alkaline condition in an aerated deep tank fermentor to obtain mildiomycin. |
