发明名称 |
DETECTION OF SPECIFIC NUCLEOTIDE IN POLYNUCLEOTIDE SAMPLE: USE OF HYBRIDISATION PROBE AND ENZYME-LABELLED MARKER |
摘要 |
A method of detecting nucleic acid sequences is disclosed and enzyme-labeled reagents for use therein. According to the invention sample polynucleotide is first hybridised with a polynucleotide probe comprising a nucleic acid sequence complementary to that which is to be detected, and having a polynucleotide tail, preferably a single stranded poly(dA) or poly(dT) tail. Following attachment of the probe to the sample, an enzyme-containing marker, is attached to the probe, the marker comprising a polynucleotide having a single-stranded portion, to the base groups of which is or are attached one or more enzymatically active groups, and a polynucleotide tail which can be attached to the probe by annealing or hybridisation. Preferably the marker has a single-stranded polynucleotide tail complementary to that on the probe whereby the marker can be attached to the probe by a second hybridisation step. Presence of the sequence to be detected is confirmed by testing the labeled sample for any enzyme related activity. |
申请公布号 |
NZ207454(A) |
申请公布日期 |
1987.05.29 |
申请号 |
NZ19840207454 |
申请日期 |
1984.03.09 |
申请人 |
MALCOLM, ALAN DAVID BLAIR;NICOLAS, JEAN LESLEY |
发明人 |
MALCOLM, ALAN DAVID BLAIR;NICOLAS, JEAN LESLEY |
分类号 |
G01N33/50;C07H21/00;C12N15/00;C12N15/09;C12Q1/68;C12Q1/70;G01N33/58 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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