Enzyme-immobilized membrane and process for producing the same.

摘要

An enzyme-immobilized membrane for use in an enzyme reaction comprises an anisotropic ultrafiltration membrane, e.g. a polysulfone, having a porous layer and a dense layer. The porous layer retains a water-soluble polymer having at least two functional groups in the crosslinked state e.g. a polyalkyleneimine, a polyalkyleneglycol, a polyamino acid or a polyallylamine, and the enzyme is covalently bonded to the membrane through the functional groups of the polymer.
<??>The enzyme-immobilized membrane is produced by impregnating a solution of the soluble polymer into the porous layer of the membrane under a pressure of 0.1 to 1.0 kg/cm<2>, washing the porous layer, passing a solution of a crosslinking agent through the porous layer under said pressure to crosslink the polymer, removing the non-crosslinked water-soluble polymer by back washing from the dense layer of the membrane, and then passing a solution of the enzyme under a pressure within the same pressure range as above through the porous layer, whereby the enzyme is covalently bonded to the membrane through the functional groups of the polymer.
<??>The enzyme can be for hydrolysing polysaccharide or protein.