发明名称 |
NEW PROCESS FOR THE DISRUPTION OF BACTERIA CELLS CONTAINING RECOMBINANT POLYPEPTIDES |
摘要 |
1. Process for isolating a polypeptide prepared by recombinant methods from bacterial cells, characterised in that bacterial cells containing a polypeptide prepared by genetic engineering, these cells being suspended in an aqueous acid at a pH of from 2.0 to 4.0, are mechanically destroyed by shear forces and subsequently the polypeptide now present in the resulting suspension is separated and further purified by known methods. |
申请公布号 |
ZA8506640(B) |
申请公布日期 |
1987.05.27 |
申请号 |
ZA19850006640 |
申请日期 |
1985.08.30 |
申请人 |
BOEHRINGER INGELHEIM INTERNATIONAL GMBH. |
发明人 |
DR. GUENTER ADOLF;PROF. DR. GERHARD BODO;WOLFGANG GIBLEY;KURT KONOPITZKY |
分类号 |
C12N15/09;A61K38/21;C07K1/14;C07K14/52;C07K14/555;C07K14/56;C12N1/00;C12N1/06;C12N1/16;C12N1/20;C12N15/00;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|