发明名称 |
Human hybridomas, precursors and products |
摘要 |
Human monoclonal antibody compositions, human-human monoclonal hybridoma cells, human myeloma cells, human antibody genes and their uses. Human myeloma cells are developed for fusing with immunized lymphoid cells to provide stable human-human hybridoma strains producing complete monoclonal antibodies for a predefined antigen. From a myeloma cell line, rapidly growing 8-azaguanine resistant HAT sensitive cells are selected. The selected myeloma cells are crossed with immunized lymphoid cells and the resulting cell mixture grown under controlled selective conditions. After expansion of the desired hybridoma cells, the monoclonal antibodies may be harvested. The hybridomas serve as a source for messenger RNA for light and heavy chains which may be used for production of light and heavy chain immunoglobulin proteins through hybrid DNA techniques.
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申请公布号 |
US4668629(A) |
申请公布日期 |
1987.05.26 |
申请号 |
US19840599201 |
申请日期 |
1984.04.09 |
申请人 |
THE BOARD OF TRUSTEES OF LELAND STANFORD JR. UNIVERSITY |
发明人 |
KAPLAN, HENRY S.;OLSSON, LENNART |
分类号 |
A61K39/395;C07K16/00;C12N5/16;(IPC1-7):C12N5/00;C12N15/00 |
主分类号 |
A61K39/395 |
代理机构 |
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代理人 |
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地址 |
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