| 摘要 |
<p>NEW MATERIAL:A lymphotoxin (LT-K) expressed by formula I (X is formula II, Met or H; Y is saccharide chain or H). USE:An antitumor agent. PREPARATION:Animal cultivated cells or microorganisms, e.g. bacteria, Escherichia coil or yeast, transformed by an expression vector of DNA sequence containing DNA sequence coding a polypeptide expressed by formula I, e.g. chromosome DNA sequence of normal human cells, and promoter sequence functioning in animal cultivated cells, e.g. promoter sequence of early gene of SV40, thymidine kinase gene of herpes simplex virus (HSV-1) or late gene of SV40, are cultivated to produce an antitumor substance, which is collected. The LT-K acts as a cell lethal factor against L929 cells (CCL-1). The action thereof is as follows; The LT-K has lethal activity against cancerous cells synergistically with human interferon-gamma.</p> |
