Matrix-bound crown ether ligands as separating material in the affinity chromatography of nucleic acids

摘要

Crown ethers or cryptands are covalently bound to a support polymer conventional in chromatography. In this manner, a chromatographic separation material is obtained which is able to reversibly bind nucleic acids and oligonucleotides in the presence of alkali metal ions. The separation material is outstandingly suitable for affinity chromatography of nucleic acids, separating off nucleic acids from biological samples and the separation of nucleic acid mixtures and oligonucleotide mixtures both on an analytical and on a preparative scale. When suitable support polymers are used, the use of the novel separation material is possible without problem in the high-pressure liquid chromatography.