PRODUCTION OF PHYSIOLOGICALLY ACTIVE SUBSTANCE AND NOVEL SERUM-FREE MEDIUM

摘要

PURPOSE:To culture an animal cell in high efficiency and produce a physiologically active substance, by adding a novel protein produced by a myeloleukemia cell strain K562-T1 to a medium in place of serum. CONSTITUTION:Human myeloleukemia cell strain K562-T1 is cultured in a medium. A novel protein substance accumulated in the culture product is separated therefrom and is added to a medium in place of serum. An animal-originated cell (B-lymphocyte cell such as Namalva, LukII, etc., T-lymphocyte cell such as CCRF-CEM, epithelial cell such as KB, myeloma cell such as SK-Ly-18, RPMI-8226, etc., monocyte cell such as HL-60, etc.) is cultured in the above medium to accumulate a physiologically active substance in the culture product.