| 摘要 |
The major iron-regulated protein of a pathogenic species of the genus Neisseria, the gonococcal congener of which (MIRP) protein has a molecular weight of approximately 37,000 daltons and consisting of about 340 amino acids, is isolated in an immunospecific antigenically substantially pure form suitable for use as a vaccine against the pathogenic species of the genus Neisseria effective to stimulate the production of a protective level of antibodies to the pathogenic species in a susceptible host, by the steps of: growing cells of that pathogenic species in an iron-depleted bacteriologic media; harvesting the thus-grown cells; disrupting the cells; separating the soluble portion of the cells from the insoluble portion; and selectivity solubilizing the MIRP from the insoluble portion of the disrupted cells with an aqueous cationic surfactant aqueous media, e.g., cetyltrimethylammonium bromide; and removing residual contaminates in the MIRP fraction by chromatographic fractionation. |
