| 摘要 |
PURPOSE:A microorganism in Saccharomyces is transformed with a specific recombinant DNA and the resultant strain is cultured to mass-produce amylase stably in high efficiency. CONSTITUTION:A DNA segment coding amylase biosynthesis (A) is obtained from cell bodies of cultured Saccharomyces fibuligera. Then, vector DNA such as plasmid pY11 (B) is obtained from Escherichea coli and Yeast. Further, Escherichea coli PR1 is transformed with a reaction product between components A and B to obtain a recombinant DNA. Finally, Saccharomyces cerevisiae 3654-1D is transformed with the resultant recombinant DNA to give a train having a plasmid, pSfalpha1 or pSfGlu1 including alpha-amylase or glucoamylase (C). The strain (C) is aerobically cultured in a medium containing carbon, nitrogen sources and other nutrients at 5-6pH and 28-35 deg.C for 5-90hr to produce amylase, which is collected. |
