发明名称 |
Purification method for proteins. |
摘要 |
<p>Tumor necrosis factor (TNF) may be purified using a hydrophobic support in a chromatographic column that is then developed, preferably using high pressure liquid chromatography. Also, recombinantly produced biologically active proteins may be purified by passing a solution containing the protein through a continuous porous hydrophobic membrane, and recovering the fraction enriched in the desired protein. TNF and recombinant ricin toxin A chain may be purified. A highly purified TNF comprising at least 95% TNF, as determined by SDS-PAGE analysis, with an endotoxin content of less than 0.1 nm/mg TNF that is substantially free of pyrogens by the USP rabbit pyrogen test at a dosage range of 1.0 to 2.4 x 10<5> U/Kg is obtained.</p> |
申请公布号 |
EP0220966(A2) |
申请公布日期 |
1987.05.06 |
申请号 |
EP19860308484 |
申请日期 |
1986.10.30 |
申请人 |
CETUS ONCOLOGY CORPORATION |
发明人 |
LIN, LEO SHUN-LEE;DORIN, GLENN;YAMAMOTO, RALPH;HANISCH, WOLFGANG HELMUT;THOMSON, JAMES WILLIAM;WOLFE, SIDNEY NORMAN |
分类号 |
C07K1/36;C07K14/525 |
主分类号 |
C07K1/36 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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