METHOD AND DEVICE FOR DETERMINATION OF ANTITHROMBIN-III

摘要

Disclosed is a method and device for the quantitative determination of AT-III in mammalian blood plasma. The method involves contacting a plasma sample containing excess heparin with a 3 layered reagent strip comprising: i. a first upper layer of a bibulous material containing thrombin and a buffer; ii. a second lower layer of a bibulous material containing a fluorogenic or chromogenic substrate capable of interacting with thrombin in a time related chemical manner; and iii. a bottom layer of water impermeable material. The chemical change in the strip is determined as a function of time and this change is compared with that obtained in samples containing known amounts of AT-III.