PROCESS FOR PREPARING FREEZE-DRIED MICROORGANISM CONCENTRATES

摘要

The process for preparing concentrated amounts of lyophilized micro-organisms employs an ultrafiltration with a supply of nutritive substrates and the supply of a neutralizing agent in the fermenter, and further comprises the following steps: (a) ceasing to supply substrates while cooling; (b) continuing the ultrafiltration until the obtainment of a primary concentrate (40-80 kg dry weight of bacteria/cu. m) and cooling to 15 DEG -20 DEG C.; (c) washing the primary concentrate with mineral salts and amino acids, oligo-elements and vitamins; (d) concentrating by ultrafiltration to 90-220 kg dry weight of bacteria/cu. m; (e) cooling to 5 DEG C. and adding a protector; (f) deep freezing and lyophilizing. The process particularly applies to the preparation of lactic bacteria.