| 摘要 |
Process for the determination of the activity of the DNAases in biological fluids. The biological fluid to be examined is added to a reaction medium consisting of a buffer solution, a soluble magnesium salt, a soluble calcium salt and deoxyribonucleic acid; the mixture thus obtained is kept for 45 to 90 minutes at a temperature of 40 to 63 DEG C and is then denatured, the insoluble substances are separated off and the remaining liquid is subjected to a spectrophotometric measurement at 260 nm in comparison with a reference solution. The process can be applied especially to the diagnosis of cancer and to the assessment of the effectiveness of the therapeutics applied to its treatment. |
